Journal: The Journal of physiology

Article Title: Diet-induced obesity in mice increases carotid body chemosensitivity via the leptin-TRPM7 pathway

doi: 10.1113/JP288722

Figure Lengend Snippet: A and B are representative illustrations of CB-CSN responses to a Trpm7 blocker, FTY720 (3 μM) during normoxia (Nx) and hypoxia (Hx) in lean ( N = 7) and diet-induced obese (DIO, N = 5) mice. C , summary data are expressed as box-and-whisker plots (median, 25%–75% centiles and minimum and maximum values). CSN responses were normalized to baseline normoxia, indicated by the dashed line. Data were statistically compared using two-way repeated-measures ANOVA: hypoxia vs . normoxia, no FTY vs . FTY720 within subjects, lean vs . DIO between subjects. Sphericity was assessed using Mauchly’s test and found to be violated. After applying Greenhouse–Geisser and Huynh–Feldt corrections, both effects of DIO and hypoxia remain significant. FTY720 had no significant effects on CSN activities during Nx and Hx in lean and DIO mice as indicated by the P -values in the figure.

Article Snippet: A total of 12 DIO Trpm7 flox mice (strain # 018784; The Jackson Laboratory; 18 weeks, body weight of 45–50 g) were used for adenovirus injection in the CB.

Techniques: Whisker Assay

Journal: The Journal of physiology

Article Title: Diet-induced obesity in mice increases carotid body chemosensitivity via the leptin-TRPM7 pathway

doi: 10.1113/JP288722

Figure Lengend Snippet: A and B , upper panels represent carotid body sections immunolabeled for Trpm7 receptors (green), type I glomus cell marker tyrosine hydroxylase (TH; red) and type II cell marker glial fibrillary acidic protein (GFAP; white) in lean ( A ) and DIO ( B ) mice. The lower panels represent merged images (co-localization) of Trpm7, TH and GFAP. C , a significant difference was observed in Trpm7 receptor protein expression between lean and DIO CBs ( P = 0.047; Mann–Witney U test). N = 6 sections from two lean mice and N = 9 sections from three DIO mice.

Article Snippet: A total of 12 DIO Trpm7 flox mice (strain # 018784; The Jackson Laboratory; 18 weeks, body weight of 45–50 g) were used for adenovirus injection in the CB.

Techniques: Immunofluorescence, Expressing, Immunolabeling, Marker

Journal: The Journal of physiology

Article Title: Diet-induced obesity in mice increases carotid body chemosensitivity via the leptin-TRPM7 pathway

doi: 10.1113/JP288722

Figure Lengend Snippet: Confocal images showing Ad-Cre-GFP expression, suggesting successful transfection of the Ad-Cre-GFP viruses into the CB of diet-induced obese (DIO) Trpm7 flox mice ( N = 2). B , summary data showing relative expression ratio (RER) of Trmp7 mRNA in the carotid bodies of lean Trmp7 flox mice treated with control Ad-GFP ( N = 6) and Ad-Cre-GFP viruses ( N = 4). There was a significant decrease in the Trpm7 mRNA expression in the Ad-Cre-GFP group ( P = 0.002; Mann–Witney U test).

Article Snippet: A total of 12 DIO Trpm7 flox mice (strain # 018784; The Jackson Laboratory; 18 weeks, body weight of 45–50 g) were used for adenovirus injection in the CB.

Techniques: Expressing, Transfection, Control

Journal: The Journal of physiology

Article Title: Diet-induced obesity in mice increases carotid body chemosensitivity via the leptin-TRPM7 pathway

doi: 10.1113/JP288722

Figure Lengend Snippet: A and B are representative illustrations of ex vivo CSN responses from diet-induced obese Trpm7 flox mice transfected into the CB with Ad-Cre-GFP and control Ad-GFP viruses, respectively. CSN responses were normalized to activity in hyperoxia (Hypx, PO 2 = 200 Torr, represented by the horizontal dashed line). Genetic knockdown of Trpm7 receptors completely abolished the stimulatory effect of leptin on CSN activity ( A ), whereas the leptin’s effect was intact when the Trpm7 channels were functional. B , the P -values for the effect of Trpm7 knockdown are shown in the figures C and D . C , summary data of basal CSN activity: normoxia (Nx) + Ad-Cre-GFP vs . Nx + leptin + Ad-Cre-GFP ; P = 0.148 ( N = 5), Nx + Ad-GFP vs . Nx + leptin + Ad-GFP ; P = 0.001 ( N = 5). D , the hypoxic chemoreflex represented as ΔCSN activity (Hx–Nx): Ad-Cre-GFP vs. Ad-Cre-GFP + leptin; P = 1.000, Ad-GFP vs. Ad-GFP + leptin; P < 0.001, Ad-Cre-GFP + leptin vs. Ad-Cre-GFP + leptin + FTY720; P = 0.456; Ad-GFP + leptin vs. Ad-GFP + leptin + FTY720; P = 0.001 ( N = 5). There was a statistically significant interaction between the Ad-GFP vs. Ad-Cre-GFP treatment groups and leptin treatment at hypoxic condition; P = 0.006. Data were compared using two-way repeated-measures ANOVA: no leptin vs . leptin vs . leptin + FTY720 within subjects, Ad-Cre-GFP vs . control Ad-GFP viruses between subjects. Sphericity was assessed using Mauchly’s test in both C and D and found not to be violated. All values are expressed as box-and-whisker plots (median, 25%–75% centiles and minimum and maximum values).

Article Snippet: A total of 12 DIO Trpm7 flox mice (strain # 018784; The Jackson Laboratory; 18 weeks, body weight of 45–50 g) were used for adenovirus injection in the CB.

Techniques: Knockdown, Activity Assay, Ex Vivo, Transfection, Control, Functional Assay, Whisker Assay